Trypanosomatid hydrogen peroxidase metabolism

FEBS Letters

Kennedy, W.P.K.¹  Sartorelli, A.C.²  Penketh, P.G.²  Patton, C.L.² 

[1] Wolfson Molecular Biology Unit, London School of Hygiene and Tropical Medicine, Keppel Street, London WCLE 7HT, England;>MacArthur Center for Parasitology and Tropical Medicine, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06510, USA

关键词: Hydrogen peroxide;    Trypanothione;    Macrophage;    (Trypanosoma;    Leishmania);    BSA;    bovine serum albumin;    NEM;    N-ethylmaleimide;    GSSG/GSH;    oxidized and reduced glutathione;    respectively;    NADH;    reduced nicotinamide adenine dinucleotide;    NADP+/NADPH;    oxidized and reduced nicotinamide adenine dinucleotide phosphate;    respectively;    TS2/T(SH)2;    oxidized and reduced trypanothione;    respectively;   

DOI  :  10.1016/0014-5793(87)80968-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The rate of whole cell H₂O₂ metabolism in several salivarian and stercorarian trypanosomes and Leishmania species was measured. These cells metabolized H₂O₂ at rates between 2.3 and 48.2 nmol/10⁸ cells per min depending upon the species employed. H₂O₂ metabolism was largely insensitive to NaN₃, implying that typical catalase and peroxidase haemoproteins are not important in H₂O₂ metabolism. The metabolism of H₂O₂, however, was almost completely inhibited by N-ethylmaleimide. In representative species, H₂O₂ metabolism was shown to occur through a trypanothione-dependent mechanism.

【 授权许可】

Unknown