Transmembrane potential variations accompanying the PMA‐triggered O− 2 and H2O2 release by mouse peritoneal macrophages

FEBS Letters

Tenu, Jean-Pierre¹  Petit, Jean-François¹  Lepoivre, Michel¹ 

[1] Institut de Biochimie, Université Paris-Sud, 91405 Orsay, France

关键词: Macrophage;    Transmembrane potential;    Superoxide anion;    Hydrogen peroxide;    Extracellular Ca2+;    di-I-C1-(5);    3;    3'-dimethylindodicarbocyanine iodide;    DMSO;    dimethylsulfoxide;    EGTA;    ethyleneglycol-bis (β-aminoethyl ether) N;    N-tetraacetic acid;    FCS;    fetal calf serum;    HBSS;    Hanks' balanced salt solution;    MEM;    minimum essential medium;    PDC;    peritoneal exudate cells;    PMA;    phorbol myristate acetate;    TPMP;    triphenylmethyl phosphonium;   

DOI  :  10.1016/0014-5793(82)81107-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Stimulation by PMA of Streptococci-elicited macrophages induced a transient membrane depolarization preceding the onset of detectable O⁻ ₂ production. Mice-resident peritoneal macrophages were unresponsive to PMA for both activities. The PMA-triggered membrane depolarization seemed to be independent from O⁻ ₂ production because inhibition of membrane depolarization by EGTA had no effect on rates of O⁻ ₂ or H₂O₂ release and rate of antimycin A insensitive O₂ uptake by Streptococci-elicited macrophages. The portion of O₂ uptake recovered as O⁻ ₂ was found to be . The rate of O⁻ ₂ release was twice the rate of H₂O₂ production (1.1 nmol H₂O₂.min⁻.10⁶ macrophages⁻¹).

【 授权许可】

Unknown