Translocation of β crystallin in neural cells in response to stress

FEBS Letters

Coop, Audrey¹  Crabbe, M.James C¹  Wiesmann, Kirsten E.H¹ 

[1] Division of Cell and Molecular Biology, School of Animal and Microbial Sciences, The University of Reading, PO. Box 228, Whiteknights, Reading, Berkshire RG6 6AJ, UK

关键词: Greek key;    Immunoglobulin;    Lens;    Blindness;    Neuroblastoma;   

DOI  :  10.1016/S0014-5793(98)00783-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

While extralenticular expression of proteins in the α crystallin (small heat shock protein) family is well documented, that for proteins in the β/γ-superfamily is less well established. Here we show, using SDS-PAGE, Western blotting and confocal microscopy, that there is a constitutive level of β crystallin expression in mouse N1E-115 neural cells. Furthermore, upon heat shock at 43°C or 55°C, or cold shock at 30°C, β crystallin immunoreactivity translocated predominantly from the nuclear region into the cytoplasmic region of the cells. In conditions of stress, it may be important for β crystallin to be recruited into the cytoplasm to stabilise other proteins via its high β-sheet content, and/or to ensure that storage levels of cytoplasmic Ca²⁺ are maintained.

【 授权许可】

Unknown