The effect of inhibitors of arachidonic acid metabolism on proliferation and death of tumor cells

FEBS Letters

Korystov, Yu.N¹  Kublik, L.N¹  Kudryavtsev, A.A¹  Shaposhnikova, V.V¹  Narimanov, A.A¹  Orlova, O.E¹  Levitman, M.Kh¹ 

[1] Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142292, Russia

关键词: Arachidonic acid metabolism;    Apoptosis;    Proliferation;    Tumor cell;    AA;    arachidonic acid;    BPB;    4-bromophenacyl bromide;    NDGA;    nordihydroguaiaretic acid;    PLA2;    phospholipase A2;   

DOI  :  10.1016/S0014-5793(98)00757-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of inhibitors of arachidonic acid metabolism on proliferation and death of tumor P-388 cells in a broad concentration range was studied. Cell proliferation was estimated by the metaphase frequency and the proportion of cells in S phase; cell death was determined from lysis, staining of cells with trypan blue, nuclear damage, percentage of cells with subdiploid DNA and the type of DNA fragmentation. It was shown that low concentrations of phospholipase A₂ and lipoxygenase inhibitors stimulated the proliferation of P-388 cells. At higher concentrations, the inhibitors suppressed cell proliferation by blocking the G₁-S transition and induced cell death of the apoptosis type. Indomethacin, an inhibitor of cyclooxygenase, did not initiate cell death, nor did it affect the proliferation of P-388 cells at concentrations of up to 10 μM.

【 授权许可】

Unknown