Transfection of cells with basic fibroblast growth factor and Kaposi fibroblast growth factor genes induce resistance to and receptor modulation of tumor necrosis factor

FEBS Letters

Pocsik, Eva¹  Aggarwal, Bharat B.¹  Totpal, Klara¹ 

[1] Cytokine Research Laboratory, Department of Molecular Oncology, The University of Texas M.D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, TX 77030, USA

关键词: Resistance;    Tumor necrosis factor;    Fibroblast growth factor;    Proliferation;    Tumor cell;    TNF;    tumor necrosis factor;    bFGF;    basic fibroblast growth factor;    K-fgf;    kaposi sarcoma growth factor/human stomach cancer/fibroblast growth factor-5;    FBS;    fetal bovine serum;    DMEM;    Dulbecco's minimum essential medium;    PLA2;    phospholipase A2;   

DOI  :  10.1016/0014-5793(95)00945-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tumor necrosis factor (TNF) has been shown to inhibit the growth of some cell types and stimulate the proliferation of others by a mechanism that is not understood. In the present study, we investigated the effect of transfection of NIH-3T3 cells with either the basic fibroblast growth factor gene (bFGF) or the kaposi FGF gene (K-fgf) on the growth-modulatory effects of TNF. Our results show that transformation of cells with either gene leads to resistance to the growth-inhibitory effects of TNF. The K-fgf gene was found to be a more potent inducer of cellular resistance than the bFGF gene. The cellular resistance correlated with the inhibition of TNF-induced activation of phospholipase A₂ and downmodulation of TNF receptors. Overall, our results indicate that both K-fgf and bFGF play an important role in suppression of antiproliferative effects of TNF.

【 授权许可】

Unknown