Brain basic fibroblast growth factor stimulates the proliferation of rat neuronal precursor cells in vitro

FEBS Letters

Gensburger, Claire¹  Labourdette, Gérard²  Sensenbrenner, Monique¹ 

[1] Centre de Neurochimie du CNRS, 5 rue Blaise Pascal, F-67084 Strasbourg Cedex, France;INSERM U.44, 5 rue Blaise Pascal, F-67084 Strasbourg Cedex, France

关键词: Fibroblast growth factor;    Proliferation;    Stimulatory effect;    (Rat neuronal cell);   

DOI  :  10.1016/0014-5793(87)81230-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Neuronal cells from cerebral hemispheres of 13-day-old rat embryos were grown in a serum-free culture medium for 48 h, 4 or 8 days. The neuronal precursor cells proliferate for 5 days. The addition of bovine brain basic fibroblast growth factor stimulates their proliferation as determined by measurement of [¹²¹I]-iododeoxyuridine incorporation and by autoradiographic analysis after [³H]thymidine incorporation. The proliferating responsive cells were characterized as neurons by immunostaining against neurofilament proteins. Five other growth factors tested were without effect on the proliferative activity of these neuronal cells. The present results show that bFGF is mitogenic in vitro for rat neuronal precursor cells of the central nervous system.

【 授权许可】

Unknown