Single molecule biochemistry using optical tweezers

FEBS Letters

Mehta, Amit D.¹  Spudich, James A.¹  Pullen, Katherine A.¹ 

[1] Department of Biochemistry, Stanford University Medical Center, Stanford, CA 94305, USA

关键词: Optical trap;    Single molecule;    Myosin;    Kinesin;    Titin;   

DOI  :  10.1016/S0014-5793(98)00594-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The use of optical trapping to create extremely compliant mechanical probes has ushered in a new field of biological inquiry, the mechanical and kinetic study of proteins at the single molecule level. This review focuses on three examples of such study and includes methods of extracting parameters of interest from the raw data such experiments generate.

【 授权许可】

Unknown