A new opioid peptide predicted from cloned cDNAs from skin of Pachymedusa dacnicolor and Agalychnis annae

FEBS Letters

Wechselberger, Christian¹  Negri, Lucia²  Kreil, Günther¹  Severini, Cinzia² 

[1] Institute of Molecular Biology, Austrian Academy of Sciences, Billrothstrasse 11, A-5020 Salzburg, Austria;Istituto di Farmacologia, Università La Sapienza, 00185 Rome, Italy

关键词: Amphibian skin;    Opioid peptide;    Deltorphin;    Phyllomedusa;   

DOI  :  10.1016/S0014-5793(98)00545-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated a cDNA encoding a precursor of dermorphin from the skin of Pachymedusa dacnicolor. Besides four copies of this opioid peptide, the deduced sequence also contains the genetic information for a novel peptide Tyr-Ile-Phe-His-Leu-Met-Asp-NH₂. This differs from Met-deltorphin by the presence of Ile at position 2. In a related precursor from the skin of Agalychnis annae, the sequence of this peptide is in the 3′-untranslated region of the cloned cDNA. From earlier results we predict that in skin peptides the second residue is d-allo-Ile. We have synthesized this and related peptides with different d-amino acids, and determined their δ agonist activity. The peptide with d-nor-Leu binds with high affinity to δ receptors, while that with d-allo-Ile is about 100 times less active.

【 授权许可】

Unknown