Identification and characterization of two dermorphins from skin extracts of the Amazonian frog Phyllomedusa bicolor

FEBS Letters

Erspamer, Giuliana Falconieri²  Barra, Donatella¹  Simmaco, Maurizio¹  Negri, Lucia²  Kreil, Günther⁴  Mignogna, Giuseppina¹  Severini, Cinzia³ 

[1] Dipartimento di Scienze Biochimiche ‘A. Rossi Fanelli’ and Centro di Biologia Molecolare del Consiglio Nazionale delle Ricerche, Università La Sapienza, 00185 Roma, Italy;Istituto di Farmacologia Medica, Università La Sapienza, 00185 Roma, Italy;Istituto di Neurobiologia del Consiglio Nazionale delle Ricerche, 00156 Roma, Italy;Institute of Molecular Biology, Austrian Academy of Sciences, 5020 Salzburg, Austria

关键词: Amphibian skin;    Dermorphin;    Deltorphin;    Opioid peptide;    GPI;    guinea-pig intestine;    MVD;    mouse vas deferens;    DAGO;    DAGMO;    Tyr-d-Ala-Gly-Phe(Me)-Gly-ol;   

DOI  :  10.1016/0014-5793(92)80427-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Skin extracts of South American hylid frogs of the subfamily Phyllomedusinae contain dermorphins and deltorphins, opioid heptapeptides highly selective for either μ or δ receptors. In all these peptides, a d-amino acid is present in the second position. The structure of the precursors for Ala-deltorphins was recently deduced from cloned cDNAs derived from skin of Phyllomedusa bicolor (Richter et al. (1990) Proc. Natl. Acad. Sci. USA 87, 4836–4839). From the amino acid sequence of these precursors, the existence of three peptides related to dermorphin could be predicted. From methanol extracts of skin of Ph. bicolor we have isolated two of these peptides. [Lys⁷]dermorphin-OH and [Trp⁴,Asn⁷]dermorphin-OH. The biological activity of these new dermorphins and their amidated counterparts is presented.

【 授权许可】

Unknown