Characterisation of an Arabidopsis cDNA encoding a thylakoid lumen protein related to a novel `pentapeptide repeat' family of proteins

FEBS Letters

Robinson, Colin¹  Kieselbach, Thomas²  Schroder, Wolfgang P²  Mant, Alexandra¹ 

[1] Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK;Arrhenius Laboratory, Department of Biochemistry, Stockholm University, S-106 91 Stockholm, Sweden

关键词: Chloroplast;    Membrane biogenesis;    Photosynthesis;    Protein translocation;    Thylakoid lumen;   

DOI  :  10.1016/S0014-5793(98)00517-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have cloned an Arabidopsis cDNA encoding a novel thylakoid lumen protein, P17.4, that has been previously isolated from lumen extracts of spinach chloroplasts. The protein is synthesised with a bipartite presequence containing a Sec-type lumen-targeting signal peptide and the precursor protein is imported into the lumen of pea chloroplasts. The encoded protein is homologous to an Anabaena protein that is essential for correct glycolipid localisation, and is also related to at least 16 unassigned open reading frames in Synechocystis. This family of proteins is characterised by the presence of numerous pentapeptide repeats with the consensus structure AXLXX, and its members are predicted to be located in the cytosol, plasma membrane and periplasm/lumen. P17.4 is therefore the first higher plant member of an extended family of putative cyanobacterial proteins that may serve important roles in lipid transport or assembly.

【 授权许可】

Unknown