Inhibition of ascorbate peroxidase under oxidative stress in tobacco having bacterial catalase in chloroplasts

FEBS Letters

Takeda, Toru²  Yokota, Akiho¹  Yamauchi, Haruko²  Sano, Satoshi¹  Shikanai, Toshiharu¹  Shigeoka, Shigeru²  Tomizawa, Ken-Ichi¹ 

[1] Research Institute of Innovative Technology for the Earth, Kizu, Kyoto 619-0292, Japan;Department of Food and Nutrition, Faculty of Agriculture, Kinki University, Nakamachi, Nara 631-8505, Japan

关键词: Ascorbate peroxidase;    Catalase;    Drought stress;    Oxidative stress;   

DOI  :  10.1016/S0014-5793(98)00483-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To analyze the potential of the active oxygen-scavenging system of chloroplasts, we introduced Escherichia coli catalase into tobacco chloroplasts. Photosynthesis of transgenic plants was tolerant to high irradiance under drought conditions, while the wild plants suffered severe damage in photosynthesis under the same conditions. Irrespective of responses to the stress, ascorbate peroxidase was completely inactivated both in the transgenic and wild-type plants. These findings are contrary to the established idea that the ascorbate peroxidase-mediated antioxidative system protects chloroplasts from oxidative stress.

【 授权许可】

Unknown