The interacting RNA polymerase II subunits, hRPB11 and hRPB3, are coordinately expressed in adult human tissues and down‐regulated by doxorubicin

FEBS Letters

Floridi, Aristide¹  Fanciulli, Maurizio¹  Passananti, Claudio²  De Angelis, Roberta¹  Di Padova, Monica¹  Bruno, Tiziana¹  Lovari, Sarah¹ 

[1] Cell Metabolism and Pharmacokinetics Laboratory, Regina Elena Cancer Institute, Via delle Messi d'Oro 156, 00158 Rome, Italy;Istituto di Tecnologie Biomediche, CNR, Viale Marx 43, 00137 Rome, Italy

关键词: Doxorubicin;    Drug toxicity;    RNA polymerase II;    Alternative splicing;    mRNA degradation;    Two-hybrid system;    dox;    doxorubicin;    pol II;    polymerase II;    hRPB11;    human RNA polymerase II subunit 11;    hRPB3;    human RNA polymerase II subunit 3;   

DOI  :  10.1016/S0014-5793(98)00431-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We previously isolated the human RPB11 cDNA, encoding the 13.3 kDa subunit of RNA polymerase II, and demonstrated that expression of this subunit is modulated by doxorubicin. Using hRPB11 as bait in a yeast two-hybrid system, two cDNA variants encoding a second RNA polymerase II subunit, hRPB3, have now been isolated and characterized. These two hRPB3 mRNA species differed in 3′ UTR region length, the longer transcript containing the AU-rich sequence motif that mediates mRNA degradation. Both hRPB11 and hRPB3 transcripts share a similar pattern of distribution in human adult tissues, with particularly high levels in both heart and skeletal muscle, and the expression of both is down-regulated by doxorubicin as found previously for the hRPB11 subunit. Taken together, these findings suggest that the interaction between hRPB3 and hRPB11 is fundamental for their function and that this heterodimer is involved in doxorubicin toxicity.

【 授权许可】

Unknown