Development of an antigen presentation system based on plum pox potyvirus

FEBS Letters

Garcı́a, Juan Antonio²  Martı́nez-Torrecuadrada, Jorge L¹  Casal, J.Ignacio¹  Fernández-Fernández, M.Rosario² 

[1]Inmunologı́a y Genética Aplicada S.A. (INGENASA), Hermanos Garcı́a Noblejas 41 2°, 28037 Madrid, Spain

[2]Centro Nacional de Biotecnologı́a (CNB-CSIC), Campus de la Universidad Autónoma de Madrid, 28049 Madrid, Spain

关键词: Virus vector;    Plant expression system;    Potyvirus;    Vaccine;    Parvovirus;   

DOI  :  10.1016/S0014-5793(98)00429-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The development of an antigen presentation system based on the plum pox potyvirus (PPV) is here described. The amino-terminal part of PPV capsid protein was chosen as the site for expression of foreign antigenic peptides. Modifications in this site were engineered to avoid the capability of natural transmission by aphids of this PPV vector. As a first practical attempt, different forms of an antigenic peptide (single and tandem repetition) from the VP2 capsid protein of canine parvovirus (CPV) were expressed. Both chimeras are able to infect Nicotiana clevelandii plants with similar characteristics to wild-type virus and remain genetically stable after several plant passages. The antigenicity of purified chimeric virions was demonstrated, proving the suitability of this system for diagnostic purposes. Moreover, mice and rabbits immunized with chimeric virions developed CPV-specific antibodies, which showed neutralizing activity.

【 授权许可】

Unknown