A cassava common mosaic virus vector for virus-induced gene silencing in cassava

Plant Methods

Hongguang Cui¹  Xiukun Yang²  Di Sun²  Yang Liu²  He Wang²  Wentao Shen³  Peng Zhou³  Pu Yan³  Decai Tuo³  Wenbin Liao³  Xiaoying Li³ 

[1] College of Plant Protection, Hainan University, 570228, Haikou, China;Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs & Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, 571101, Haikou, China;College of Horticulture, Hainan University, 570228, Haikou, China;Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs & Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, 571101, Haikou, China;Hainan Key Laboratory for Protection and Utilization of Tropical Bioresources &, Institute for Tropical Agricultural Resources, Chinese Academy of Tropical Agricultural Sciences, 571101, Haikou, China;

关键词: Cassava;    Cassava common mosaic virus;    Virus vector;    Virus-induced gene silencing;   

DOI  :  10.1186/s13007-021-00775-w

来源: Springer

【 摘 要 】

BackgroundCassava is an important crop for food security and industry in the least-developed and developing countries. The completion of the cassava genome sequence and identification of large numbers of candidate genes by next-generation sequencing provide extensive resources for cassava molecular breeding and increase the need for rapid and efficient gene function analysis systems in cassava. Several plant virus-induced gene silencing (VIGS) systems have been developed as reverse genetic tools for rapid gene function analysis in cassava. However, these VIGS vectors could cause severe viral symptoms or inefficient gene silencing.ResultsIn this study, we constructed agroinfection-compatible infectious cDNA clones of cassava common mosaic virus isolate CM (CsCMV-CM, genus Potexvirus, family Alphaflexiviridae) that causes systemic infection with mild symptoms in cassava. CsCMV-CM was then modified to a viral vector carrying the Nimble cloning frame, which facilitates the rapid and high-throughput cloning of silencing fragments into the viral genome. The CsCMV-based vector successfully silenced phytoene desaturase (PDS) and magnesium chelatase subunit I (ChlI) in different cassava varieties and Nicotiana benthamiana. The silencing of the ChlI gene could persist for more than two months.ConclusionsThis CsCMV-based VIGS system provides a new tool for rapid and efficient gene function studies in cassava.

【 授权许可】

CC BY