Characterisation of γ‐glutamylcysteine synthethase‐heavy subunit promoter: a critical role for AP‐1

FEBS Letters

MacNee, William¹  Antonicelli, Frank¹  Rahman, Irfan¹  Smith, Christopher A.D¹ 

[1] Rayne Laboratory, Respiratory Medicine Unit, Department of Medicine, University of Edinburgh, Royal Infirmary, Lauriston Place, Edinburgh EH3 9YW, UK

关键词: Glutathione;    γ-Glutamylcysteine synthetase;    Promoter analysis AP-1;    Oxidant;    A549;   

DOI  :  10.1016/S0014-5793(98)00410-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The 5′-flanking region of human γ-glutamylcysteine synthetase-heavy subunit (γ-GCS-HS) was characterised by creating a series of chloramphenicol acetyl transferase (CAT) reporter deletion constructs. Analysis of various deleted CAT constructs revealed that a putative AP-1 consensus sequence is required to direct the constitutive and oxidant-mediated promoter activity. Gel mobility shift and mutation analysis of the sequence (−269 to −263 bp), showed binding of AP-1 is involved in the oxidant-mediated regulation of γ-GCS-HS promoter activity.

【 授权许可】

Unknown