Disruption of γ‐glutamylcysteine synthetase results in absolute glutathione auxotrophy and apoptosis in Candida albicans

FEBS Letters

Baek, Yong-Un¹  Kim, Yeon-Ran¹  Yim, Hyung-Soon¹  Kang, Sa-Ouk¹ 

[1] Laboratory of Biophysics, School of Biological Sciences, and Institute of Microbiology, Seoul National University, Seoul 151-742, South Korea

关键词: Glutathione;    γ-Glutamylcysteine synthetase;    Apoptosis;    Candida albicans;    GSH;    glutathione;    GSSG;    glutathione disulfide;    ROS;    reactive oxygen species;    FACS;    fluorescence-activated cell sorter;    TUNEL;    terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling;   

DOI  :  10.1016/S0014-5793(03)01363-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Glutathione is the most abundant non-protein thiol and a major source of reducing equivalents in eukaryotes. We examined the role of glutathione in Candida albicans by the disruption of γ-glutamylcysteine synthetase (GCS1), an essential enzyme in glutathione biosynthesis. The gcs1/gcs1 null mutants exhibited glutathione auxotrophy, which could be rescued by supplementing with reduced and oxidized glutathione and γ-glutamylcysteine. When the mutants were depleted of glutathione, they showed typical markers of apoptosis. These results suggest that glutathione itself is an essential metabolite and C. albicans lacking GCS1 undergoes apoptosis.

【 授权许可】

Unknown