Coordinate induction of glutathione biosynthesis and glutathione‐metabolizing enzymes is correlated with salt tolerance in tomato

FEBS Letters

Foyer, Christine H²  Kiddle, Guy²  Volokita, Micha³  Gómez, Leonardo²  Mittova, Valentina³  Theodoulou, Frederica L²  Guy, Micha³  Tal, Moshe¹ 

[1] Department of Life Science, Ben-Gurion University of the Negev, P.O. Box 653, Beer Sheva 84105, Israel;Crop Performance and Improvement Department, Rothamsted Research, Harpenden, Herts AL5 2JQ, UK;Albert Katz Department of Drylands Biotechnologies, Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev, Sede Boqer Campus 84990, Israel

关键词: Salt tolerance;    Tomato;    Glutathione;    γ-Glutamylcysteine synthetase;    Glutathione-S-transferase;    Glutathione peroxidase;    BSO;    buthionine sulfoximine;    γ-ECS;    γ-glutamylcysteine synthetase;    Lem;    Lycopersicon esculentum;    Lpa;    Lycopersicon pennellii;    GPX;    glutathione peroxidase;    GR;    glutathione reductase;    GSH;    reduced glutathione;    GSSG;    oxidized glutathione;    GST;    glutathione-S-transferase;    NADPH;    nicotinamide adenine dinucleotide phosphate (reduced form);    MDA;    malondialdehyde;    TBS;    Tris buffered saline;   

DOI  :  10.1016/S0014-5793(03)01214-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The acclimation of reduced glutathione (GSH) biosynthesis and GSH-utilizing enzymes to salt stress was studied in two tomato species that differ in stress tolerance. Salt increased GSH content and GSH:GSSG (oxidized glutathione) ratio in oxidative stress-tolerant Lycopersicon pennellii (Lpa) but not in Lycopersicon esculentum (Lem). These changes were associated with salt-induced upregulation of γ-glutamylcysteine synthetase protein, an effect which was prevented by preincubation with buthionine sulfoximine. Salt treatment induced glutathione peroxidase and glutathione-S-transferase but not glutathione reductase activities in Lpa. These results suggest a mechanism of coordinate upregulation of synthesis and metabolism of GSH in Lpa, that is absent from Lem.

【 授权许可】

Unknown