FEBS Letters | |
Differences in chain length distribution of inulin from Cynara scolymus and Helianthus tuberosus are reflected in a transient plant expression system using the respective 1‐FFT cDNAs | |
Heyer, Arnd G1  Gritscher, Dominique1  Raap, Maik1  Hellwege, Elke M1  Willmitzer, Lothar1  | |
[1] Max-Planck-Institut für Molekulare Pflanzenphysiologie, Karl-Liebknecht-Str. 25, D-14476 Golm, Germany | |
关键词: Fructan fructan 1-fructosyltransferase; Inulin; Fructan pattern; Artichoke; Jerusalem artichoke; DP; degree of polymerization; 1-FFT; fructan fructan 1-fructosyltransferase; 6G-FFT; fructan fructan 6G-fructosyltransferase; HPAEC; high performance anion exchange chromatography; MDP; mean degree of polymerization; 6-SFT; sucrose fructan 6-fructosyltransferase; 1-SST; sucrose sucrose 1-fructosyltransferase; WDP; weight average degree of polymerization; | |
DOI : 10.1016/S0014-5793(98)00386-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A newly isolated cDNA clone, Cy3, encoding the fructan fructan 1-fructosyltransferase (1-FFT) from artichoke was expressed using tobacco protoplasts as expression system. Analysis of the inulin molecules synthesized upon incubation of protoplast extracts with a mixture of oligofructans (DP3–5) shows the production of inulins with a degree of polymerization (DP) of up to 23, whereas parallel experiments performed using a 1-FFT cDNA from Jerusalem artichoke led to the production of fructans with a DP of up to only 12. The results of in vitro fructan synthesis catalyzed by transiently expressed enzymes therefore reflect the difference of in vivo fructan composition of Jerusalem artichoke (MDP=8–10) and artichoke (MDP=65). These data suggest that the fructan pattern in a given species is mainly defined by the enzymatic characteristics of 1-FFT.
【 授权许可】
Unknown
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