Differences in chain length distribution of inulin from Cynara scolymus and Helianthus tuberosus are reflected in a transient plant expression system using the respective 1‐FFT cDNAs

FEBS Letters

Heyer, Arnd G¹  Gritscher, Dominique¹  Raap, Maik¹  Hellwege, Elke M¹  Willmitzer, Lothar¹ 

[1] Max-Planck-Institut für Molekulare Pflanzenphysiologie, Karl-Liebknecht-Str. 25, D-14476 Golm, Germany

关键词: Fructan fructan 1-fructosyltransferase;    Inulin;    Fructan pattern;    Artichoke;    Jerusalem artichoke;    DP;    degree of polymerization;    1-FFT;    fructan fructan 1-fructosyltransferase;    6G-FFT;    fructan fructan 6G-fructosyltransferase;    HPAEC;    high performance anion exchange chromatography;    MDP;    mean degree of polymerization;    6-SFT;    sucrose fructan 6-fructosyltransferase;    1-SST;    sucrose sucrose 1-fructosyltransferase;    WDP;    weight average degree of polymerization;   

DOI  :  10.1016/S0014-5793(98)00386-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A newly isolated cDNA clone, Cy3, encoding the fructan fructan 1-fructosyltransferase (1-FFT) from artichoke was expressed using tobacco protoplasts as expression system. Analysis of the inulin molecules synthesized upon incubation of protoplast extracts with a mixture of oligofructans (DP3–5) shows the production of inulins with a degree of polymerization (DP) of up to 23, whereas parallel experiments performed using a 1-FFT cDNA from Jerusalem artichoke led to the production of fructans with a DP of up to only 12. The results of in vitro fructan synthesis catalyzed by transiently expressed enzymes therefore reflect the difference of in vivo fructan composition of Jerusalem artichoke (M_DP=8–10) and artichoke (M_DP=65). These data suggest that the fructan pattern in a given species is mainly defined by the enzymatic characteristics of 1-FFT.

【 授权许可】

Unknown