Ectopic expression of lunatic Fringe leads to downregulation of Serrate‐1 in the developing chick neural tube; analysis using in ovo electroporation transfection technique

FEBS Letters

Katsube, Ken-ichi²  Takagi, Minoru²  Takeda, Shin'ichi¹  Nakamura, Harukazu³  Sakamoto, Kei² 

[1]Department of Molecular Genetics, National Institute of Neurosciences, National Center of Neurology and Psychiatry, Tokyo 187-8502, Japan

[2]Department of Oral Pathology, Faculty of Dentistry, Tokyo Medical and Dental University, Tokyo 113-8549, Japan

[3]Department of Molecular Neurobiology, Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575, Japan

关键词: Chicken;    Lunatic Fringe;    Notch signal pathway;    Neurogenesis;    In ovo electroporation;   

DOI  :  10.1016/S0014-5793(98)00369-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Lunatic Fringe (l-Fng) is one of the vertebrate homologues of Drosophila Fringe, which interacts with the Notch signal pathway and regulates activation of the Notch ligands, Delta and Serrate. To elucidate the roles of l-Fng in vertebrate neurogenesis, we transfected chick l-Fng (C-l-Fng) to chick neural tube using the in ovo electroporation technique and examined the subsequent changes in expression of Notch-related genes. We observed downregulation of C-Serrate-1 by ectopic C-l-Fng expression which implied that C-l-Fng acts on the vertebrate Notch pathway to regulate the expression of its ligand.

【 授权许可】

Unknown