Proton exchange kinetics in [d(ACGTATACGT)]2‐echinomycin and [d(ACGTTAACGT)]2‐echinomycin complexes

FEBS Letters

Lee, Joon-Hwa¹  Park, Jin-Young¹  Choi, Byong-Seok¹ 

[1] Department of Chemistry, Korea Advanced Institute of Science and Technology, 373-1, Kusong-dong, Yusong-gu, Taejon 305-701, South Korea

关键词: Echinomycin;    Nuclear magnetic resonance;    DNA complex;    Proton exchange;    NMR;    nuclear magnetic resonance;    NOE;    nuclear Overhauser effect;    NOESY;    nuclear Overhauser effect spectroscopy;    TTAA-complex;    d(ACGTTAACGT)2-echinomycin complex;    TATA-complex;    d(ACGTATACGT)2-echinomycin complex;    TTAA-duplex;    d(ACGTTAACGT)2 duplex;    TATA-duplex;    d(ACGTATACGT)2 duplex;   

DOI  :  10.1016/S0014-5793(98)00366-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Based on imino proton exchange catalysis, base-pair lifetimes and apparent dissociation constants are reported on the complexes formed by bisintercalation of echinomycin at the CpG steps of the d(ACGTATACGT)₂ and d(ACGTTAACGT)₂ duplexes. The lifetimes of the four central A·T base pairs between two echinomycin binding sites are much shorter than in the free duplexes. The destabilization of base pairs adjacent to the binding sites is propagated one additional base pair away from the binding site.

【 授权许可】

Unknown