Direct formation of mixed micelles in the solubilization of phospholipid liposomes by Triton X‐100

FEBS Letters

Parra, Jose Luis²  Coderch, Luisa²  López, Olga²  López-Iglesias, Carmen¹  de la Maza, Alfonso²  Wehrli, Ernst³ 

[1] Servicios Cientı́fico-Técnicos, Universidad de Barcelona, C/Lluis Solé i Sabarı́s 1-3, 08028 Barcelona, Spain;Departamento de Tensioactivos, Centro de Investigación y Desarrollo (C.I.D.), Consejo Superior de Investigaciones Cientı́ficas (C.S.I.C.), C/ Jordi Girona, 18-26, 08034 Barcelona, Spain;ETH-Zentrum, Laboratory for Electron Microscopy, Universitätsstrasse 16, CH-8092 Zürich, Switzerland

关键词: Freeze-fracture electron microscopy;    Dynamic light scattering variation;    Triton X-100;    Phosphatidylcholine liposome;    Liposome solubilization;    Vesicle-micelle structural transition;    TX-100;    Triton X-100;    PC;    phosphatidylcholine;    DLS;    dynamic light scattering;    FFEM;    freeze-fracture electron microscopy;    HD;    hydrodynamic diameter;    PI;    polydispersity index;    ReSAT;    surfactant/lipid molar ratio for bilayer saturation;    ReSOL;    surfactant/lipid molar ratio for bilayer solubilization;   

DOI  :  10.1016/S0014-5793(98)00363-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The vesicle to micelle transition which results in the interaction of the Triton X-100 surfactant with phosphatidylcholine vesicles was studied by means of dynamic light scattering (at different reading angles) and by freeze-fracture electron microscopy techniques. Vesicle solubilization was produced by the direct formation of mixed micelles without the formation of complex intermediate aggregates. Thus, vesicle to micelle transformation was mainly governed by the progressive formation of mixed micelles within the bilayer. A subsequent separation of these micelles from the liposome surface (vesicle perforation by the formation of surfactant-stabilized holes on the vesicle surface) led to a complete solubilization of liposomes.

【 授权许可】

Unknown