Bioassays Against Pinewood Nematode: Assessment of a Suitable Dilution Agent and Screening for Bioactive Essential Oils

Molecules

Pedro Barbosa³  Jorge M. S. Faria²  Marta D. Mendes²  Luís Silva Dias⁴  Maria Teresa Tinoco¹  José G. Barroso²  Luis G. Pedro²  Ana Cristina Figueiredo² 

[1] ICAAM, Departamento de Química, Universidade de Évora, 7000-671 Évora, Portugal;Universidade de Lisboa, Faculdade de Ciências de Lisboa, DBV, IBB, Centro de Biotecnologia Vegetal, C2, Campo Grande, 1749-016 Lisboa, Portugal;NemaLab-ICAAM, Departamento de Biologia, Universidade de Évora, 7002-554 Évora, Portugal;Departamento de Biologia, Universidade de Évora, 7002-554 Évora, Portugal

关键词: Bursaphelenchus xylophilus;    essential oils;    nematicide activity;    acetone;    Triton X-100;   

DOI  :  10.3390/molecules171012312

来源: mdpi

【 摘 要 】

Acetone was investigated and found to be an appropriate alternative to Triton X-100 as a solvent of essential oils in bioassays aimed to investigate their effects on pinewood nematode (Bursaphelenchus xylophilus) mortality. Therefore it was used as dilution agent to screen the effectiveness of fifty two essential oils against this pest. Thirteen essential oils were highly effective, resulting in more than 90% pinewood nematode mortality at 2 mg/mL, with six of them resulting in 100% mortality. LC₁₀₀ values ranged between 0.50 mg/mL and 0.83 mg/mL for the essential oils of Origanum vulgare and Satureja montana, respectively. Essential oils were submitted to gas chromatography and gas chromatography-mass spectrometry analysis and their chemical composition established. Data from essential oils with 100% mortality at 2 mg/mL and other essential oils previously found to have LC₁₀₀ ≤ 2 mg/mL was combined, their chemical profiles investigated by correspondences analysis plus automatic classification.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.