Interaction of melittin with negatively charged phospholipids: Consequences for lipid organization

FEBS Letters

Verkleij, A.J.²  van Esch, J.H.¹  de Kruijff, B.³  Leunissen-Bijvelt, J.²  Batenburg, A.M.¹ 

[1] Department of Biochemistry, State University of Utrecht, Padualaan 8, Utrecht, The Netherlands;Department of Molecular Cell Biology, State University of Utrecht, Padualaan 8, Utrecht, The Netherland;Institute of Molecular Biology and Medical Biotechnology, State University of Utrecht, Padualaan 8, Utrecht, The Netherlands

关键词: Melittin;    Hexagonal HII phase;    Protein-lipid interaction;    31P-NMR;    Nonbilayer lipid structure;    Freeze-fracture electron microscopy;    (DO)PA;    (dioleoyl-) phosphatidic acid;    (DO)PS;    (dioleoyl-) phosphatidylserine;    (DO)PC;    (dioleoyl-)phosphatidylcholine;    PG;    phosphatidylglycerol;    R i;    phospholipid to peptide molar ratio;    SUV;    small unilamellar vesicles;   

DOI  :  10.1016/0014-5793(87)80526-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A characterization of the structural alterations induced by melittin in model-membranes of dioleoylphosphatidic acid and egg phosphatidylglycerol is presented, based on the use of ³¹P-NMR, freeze-fracture electron microscopy and small angle X-ray scattering. In accordance with earlier findings on the cardiolipinmelittin system, melittin is found to have an inverted phase inducing effect on these negatively charged lipids, in contrast to the influence on zwitterionic phospholipids. In phosphatidic acid this is expressed in the formation of an H_II phase; in phosphatidylglycerol a less ordered, non-lamellar structure with low water content is adopted.

【 授权许可】

Unknown