Secondary structure of a mitochondrial signal peptide in lipid bilayer membranes

FEBS Letters

Tamm, Lukas K.¹  Bartoldus, Ingrid¹ 

[1] Department of Biophysical Chemistry, Biocenter, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland

关键词: Mitochondrial signal peptide;    Circular dichroism;    Protein-lipid interaction;    Secondary structure;    Amphiphilic helix;    POPC;    1-palmitoyl-2-oleoyl-phosphatidylcholine;    POPG;    1-palmitoyl-2-oleoyl-phosphatidylglycerol;   

DOI  :  10.1016/0014-5793(90)80441-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The secondary structure of the synthetic signal peptide of cytochrome c oxidase subunit IV (coxIV-25) has been measured by circular dichroism spectroscopy in different lipid environments. CoxIV-25 is polymorphic in membranes. It forms an amphiphilic α-helix both in negatively charged lipid bilayers (up to 49% helix) and in detergent micelles (up to 42% helix). In associaton with bilayers of the zwitterionic lipid phosphatidylcholine, coxIV-25 takes an aperiodic, unidentified structure. CoxIV-25 is also partially α-helical in bilayers of cardiolipin, mitochondrial lipid extracts and mixtures of synthetic phosphatidylcholine and phosphatidylglycerol.

【 授权许可】

Unknown