Further assignment of resonances in 1H NMR spectra of cerebrospinal fluid (CSF)

FEBS Letters

Maillet, Serge¹  Cozzone, Patrick J¹  Nicoli, François¹  Lutz, Norbert W¹  Viout, Patrick¹ 

[1] Centre de Résonance Magnétique Biologique et Médicale, UMR CNRS 6612, Faculté de Médecine, 27 bd Jean Moulin, 13005 Marseille, France

关键词: 1H NMR spectroscopy;    Biofluid;    Cerebrospinal fluid;    Signal assignment;    AAB;    α-aminobutyrate;    AHB;    α-hydroxybutyrate;    AHIV;    α-hydroxyisovalerate;    AOIV;    α-oxoisovalerate;    AQ;    acquisition time;    BHB;    β-hydroxybutyrate;    BHIV;    β-hydroxyisovalerate;    Cit;    citrate;    COSY;    correlation spectroscopy;    CSF;    cerebrospinal fluid;    CW;    continuous wave;    FID;    free induction decay;    FT;    Fourier transform;    HPLC;    high-performance liquid chromatography;    JRES;    J-resolved spectroscopy;    Lac;    lactate;    LB;    line broadening;    NMR;    nuclear magnetic resonance;    1;    2pd;    1;    2-propanediol;    PW;    pulse width;    RD;    relaxation delay;    S/N;    signal-to-noise ratio;    SW;    spectral width;    TD;    time domain;    TR;    repetition time;    TSP;    sodium 2;    2′;    3;    3′-tetradeutero-3-trimethylsilylpropionate;    U;    unassigned doublet;    UV;    ultraviolet;    VTU;    variable temperature unit;    X;    unassigned singlet;   

DOI  :  10.1016/S0014-5793(98)00262-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A number of previously unidentified ¹H NMR signals detected in CSF spectra of patients with various neurological and metabolic diseases are assigned to metabolites, drugs and drug excipients. Two-dimensional ¹H NMR spectroscopy (COSY and J-resolved) is employed to resolve resonances which are hidden by superimposed peaks in one-dimensional spectra. Assignments obtained by making use of 2-D techniques, and of a 1-D ¹H NMR data base created for ca. 150 authentic compounds, enable us to clarify the nature of complex signal patterns found in crowded spectral regions of CSF such as the aliphatic methyl region at ca. 1.0 ppm.

【 授权许可】

Unknown