Glucagon‐like peptide‐1 receptor expression in Xenopus oocytes stimulates inositol trisphosphate‐dependent intracellular Ca2+ mobilization

FEBS Letters

Gromada, Jesper¹  Anker, Charlotte²  Wahl, Philip²  Bokvist, Krister¹  Knudsen, Lotte B² 

[1] Department of Islet Cell Physiology, Novo Nordisk A/S, Symbion, Fruebjergvej 3, DK-2100 Copenhagen, Denmark;Department of Molecular Pharmacology, Novo Nordisk A/S, Copenhagen, Denmark

关键词: Glucagon-like peptide-1;    Calcium;    Inositol trisphosphate;    Xenopus oocyte;    Cyclic adenosine monophosphate;    [Ca2+]i;    intracellular free Ca2+ concentration;    GLP-1;    glucagon-like peptide-1;    GLP-1(7–36)amide;    glucagon-like peptide-1(7–36)amide;    Ins(1;    4;    5)P3;    inositol 1;    4;    5-trisphosphate;    PKA;    protein kinase A;    PACAP;    pituitary adenylate cyclase-activating polypeptide;    PLC;    phospholipase C;    VIP;    vasoactive intestinal peptide;   

DOI  :  10.1016/S0014-5793(98)00254-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The signal transduction pathway of the cloned human glucagon-like peptide-1 (GLP-1) receptor was studied in voltage-clamped Xenopus oocytes. Binding of GLP-1(7–36)amide was associated with cAMP production, increased [Ca²⁺]_i and activation of Ca²⁺-dependent Cl⁻ current. The effect of GLP-1(7–36)amide reflects intracellular Ca²⁺ mobilization and was suppressed by injection of the Ca²⁺ chelator BAPTA and the inositol trisphosphate receptor antagonist heparin. The responses were not mimicked by the adenylate cyclase activator forskolin and unaffected by the protein kinase A (PKA) inhibitor Rp-cAMPS. We conclude that GLP-1 receptor expression in Xenopus oocytes evokes inositol trisphosphate-dependent intracellular Ca²⁺ mobilization independent of the cAMP/PKA signaling pathway.

【 授权许可】

Unknown