A mammalian sperm cytosolic phospholipase C activity generates inositol trisphosphate and causes Ca2+ release in sea urchin egg homogenates

FEBS Letters

Jones, Keith T¹  Parrington, John¹  Swann, Karl¹  Cruttwell, Caroline¹ 

[1] Department of Anatomy and Developmental Biology, University College, Gower Street, London WC1E 6BT, UK

关键词: Egg;    Sperm;    Calcium;    Inositol trisphosphate;    Phospholipase C;    bsf;    boar sperm factor;    cADPR;    cyclic ADPRibose;    IM;    intracellular medium;    InsP3;    inositol 1;    4;    5-trisphosphate;    InsP3R;    InsP3 receptor;    NAADP;    nicotinic acid adenine dinucleotide phosphate;    PI;    phosphatidylinositol;    PIP2;    phosphatidyl inositol 4;    5-bisphosphate;    PLC;    phospholipase C;    PI-PLC;    PI-specific PLC;    PIP2-PLC;    PIP2 specific PLC;    RyR;    ryanodine receptor;   

DOI  :  10.1016/S0014-5793(98)01254-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Injection of sperm extracts triggers Ca²⁺ oscillations in mammalian eggs similar to those seen at fertilisation. Here, we show that addition of sperm extracts to sea urchin egg homogenates causes Ca²⁺ release and inositol 1,4,5-trisphosphate (InsP₃) production. Furthermore depleting homogenates of phosphatidylinositol lipids using a phosphatidylinositol-specific phospholipase C blocked the sperm extract from causing InsP₃ production and a Ca²⁺ rise. A response could be recovered by the addition of phosphatidylinositol 4,5-bisphosphate to either sperm extracts or egg homogenates. These data indicate that sperm extracts contain an InsP₃-generating phospholipase C which may play a role in Ca²⁺ release at fertilisation.

【 授权许可】

Unknown