Apolipoprotein E, transthyretin and actin in the CSF of Alzheimer's patients: relation with the senile plaques and cytoskeleton biochemistry

FEBS Letters

Aguillon, Dominique³  Merched, Aksam³  Faure, Gilbert²  Visvikis, Sophie³  Serot, Jean-Marie¹  Siest, Gérard³ 

[1] Hôpital Hotel Dieu, 54350 Mont Saint-Martin, France;CRIP, Centre de Recherche en Immuno-Pathologie, Faculté de Médecine de Nancy, BP 184, F-54500 Vandoeuvre-lès-Nancy, France;Centre de Médecine Préventive, UPRES, 2, rue du Doyen Jacques Parisot BP 7, F-54501 Vandoeuvre-lès-Nancy, France

关键词: Transthyretin;    Apolipoprotein E;    Actin;    Cerebrospinal fluid;    Senile plaque;    Alzheimer's disease;    2D-PAGE;    two-dimensional polyacrylamide gel electrophoresis;    Aβ;    β-amyloid peptide;    AD;    Alzheimer's disease;    Apo;    apolipoprotein;    CNS;    central nervous system;    CSF;    cerebrospinal fluid;    SP;    senile plaques;    TTR;    transthyretin;   

DOI  :  10.1016/S0014-5793(98)00234-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We measured the levels of two β-amyloid (Aβ)-sequestering proteins, apolipoprotein (Apo) E and transthyretin (TTR), in ventricular human cerebrospinal fluid (CSF) of Alzheimer's disease (AD) patients and controls in relation to brain histological findings. We also studied actin levels in CSF as a marker of the biochemical role of these two proteins in the cytoskeleton. We show that TTR levels in CSF were significantly decreased in AD patients compared to controls and negatively correlated with the senile plaque (SP) abundance. Moreover, actin levels were positively linked to TTR levels and increased in CSF samples of patients homozygous for the ApoE ϵ4-allele. We propose that TTR and ApoE4 may have competition in the aggregation of Aβ and its deposition in the SP of AD brain. The relationships between ApoE, TTR and actin could suggest a metabolic implication of ApoE genetics and TTR levels in cytoskeletal biochemistry which may be relevant to the pathogenesis of AD.

【 授权许可】

Unknown