On the target of a novel class of antibiotics, oxazolidinones, active against multidrug‐resistant Gram‐positive bacteria

FEBS Letters

Burghardt, Helena¹  Müller, Matthias¹  Schimz, Karl-Ludwig² 

[1] Adolf Butenandt Institut für Physikalische Biochemie, Ludwig-Maximilians-Universität München, Schillerstrasse 44, D-80336 Munich, Germany;Institut für Biotechnologie, Forschungszentrum Jülich, D-52425 Jülich, Germany

关键词: Antibiotic;    Gram-positive bacterium;    Oxazolidinone;    Ribosomal subunit;    Translation initiation;   

DOI  :  10.1016/S0014-5793(98)00194-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Oxazolidinones are a promising new class of synthetic antibiotics active against multidrug-resistant Gram-positive bacteria. To elucidate their mode of action, the effect of DuP 721 on individual steps of protein translation was studied. The drug does not interfere with translation initiation at the stage of mRNA binding or formation of 30S pre-initiation complexes. However, it inhibits the puromycin-mediated release of [³⁵S]formyl-methionine from 70S initiation complexes in a dose-dependent manner. Inhibition involves binding of the oxazolidinone to the large ribosomal subunit and is twice as high with 50S subunits from Gram-positive as with those from Gram-negative bacteria.

【 授权许可】

Unknown