Regulation of a plant plasma membrane sucrose transporter by phosphorylation

FEBS Letters

Delrot, Serge¹  Bonmort, Janine¹  Roblin, Gabriel¹  Sakr, Soulaiman¹ 

[1] ERS CNRS 6099, Laboratoire de Physiologie et Biochimie Végétales, Bâtiment Botanique, 40 Avenue du Recteur Pineau, 86022 Poitiers Cedex, France

关键词: Assimilate transport;    Membrane phosphorylation;    Okadaic acid;    Plant;    Plasma membrane;    Sucrose transporter;    AC;    accession code;    CCCP;    carbonyl cyanide m-chlorophenylhydrazone;    DMSO;    dimethylsulfoxide;    MOA;    methyl okadaic acid;    OA;    okadaic acid;    PM;    plasma membrane;    pmf;    proton-motive force;    PMV;    plasma membrane vesicles;   

DOI  :  10.1016/S0014-5793(98)00165-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The protein phosphatase inhibitor okadaic acid (OA) either provided directly to sugar beet (Beta vulgaris L.) leaf discs or infiltrated in the leaf blade rapidly inhibited sucrose uptake. Methyl okadaic acid, a biologically inactive analogue of OA, had only a marginal effect on uptake. OA inhibited proton-motive force-driven uptake of sucrose into plasma membrane vesicles, without affecting their proton permeability. OA did not significantly affect the amount of sucrose transporters present in the vesicles, as estimated by ELISA with specific antibodies. It is concluded that OA directly inhibits the activity of a H⁺-sucrose cotransporter of the plant plasma membrane, likely by maintaining it in a phosphorylated form.

【 授权许可】

Unknown