Receptor‐like kinase activity in membranes of Arabidopsis thaliana

FEBS Letters

Bleecker, Anthony B.¹  Schaller, G.Eric¹ 

[1] Department of Botany, 430 Lincoln Drive, University of Wisconsin, Madison, WI 53706-1381, USA

关键词: Receptor protein kinase;    Membrane phosphorylation;    Signal transduction;    Arabidopsis thaliana;   

DOI  :  10.1016/0014-5793(93)80676-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A class of protein kinases in the plant Arabidopsis thaliana has been identified which has biochemical characteristics similar to those of the animal receptor protein kinases. These plant protein kinases are membrane-associated, glycosylated, exhibit a preference for Mn²⁺ relative to Mg²⁺, and range from 115 to 135 kDa when identified by renaturation after protein blotting. Evidence is presented that the TMK1 protein, a receptor-like protein kinase identified in Arabidopsis from its cloned gene, is also glycosylated in its native form, and shows a preference for Mn²⁺ over Mg²⁺ when assayed for kinase activity. All of the plant receptor-like protein kinases identified phosphorylate only serine and threonine residues.

【 授权许可】

Unknown