The yeast gene YJR025c encodes a 3‐hydroxyanthranilic acid dioxygenase and is involved in nicotinic acid biosynthesis

FEBS Letters

Rytka, Joanna¹  Kucharczyk, Roza¹  Herbert, Christopher J²  Zagulski, Marek¹ 

[1] Institute of Biochemistry and Biophysics, Polish National Academy of Sciences, 5a Pawinskiego St., 02-106 Warsaw, Poland;Centre de Génétique Moléculaire, Laboratoire propre du CNRS associé à l'Université Pierre et Marie Curie, 91198 Gif-sur-Yvette, France

关键词: 3-Hydroxyanthranilic acid dioxygenase;    Kynurenine pathway;    Nicotinic acid biosynthesis;    Tryptophan degradation;    Saccharomyces cerevisiae;   

DOI  :  10.1016/S0014-5793(98)00153-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have deleted the yeast gene YJR025c and shown that this leads to an auxotrophy for nicotinic acid. The deduced protein sequence of the gene product is homologous to the human 3-hydroxyanthranilic acid dioxygenase (EC 1.13.11.6) which is part of the kynurenine pathway for the degradation of tryptophan and the biosynthesis of nicotinic acid. In cell-free extracts the 3-hydroxyanthranilic acid dioxygenase activity is proportional to the copy number of the YJR025c gene. As YJR025c encodes the yeast 3-hydroxyanthranilic acid dioxygenase, we have named this gene BNA1 for iosynthesis of icotinic cid.

【 授权许可】

Unknown