Aerobic and anaerobic NAD+ metabolism in Saccharomyces cerevisiae

FEBS Letters

Rytka, Joanna¹  Suski, Catherine²  Kucharczyka, Roza¹  Nawara, Magdalena¹  Panozzo, Cristina²  Herbert, Christopher J²  Skoneczny, Marek¹  Bécam, Anne-Marie² 

[1] Institute of Biochemistry and Biophysics, Polish National Academy of Sciences, 5a Pawinskiego, 02-106 Warsaw, Poland;Centre de Génétique Moléculaire, Laboratoire propre du CNRS, associé à l'Université Pierre et Marie Curie, F-91198 Gif-sur-Yvette, France

关键词: Aerobic;    Anaerobic;    NAD+ metabolism;    Tryptophan degradation;    NPT1;    Saccharomyces cerevisiae;   

DOI  :  10.1016/S0014-5793(02)02585-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In Saccharomyces cerevisiae the nicotinic acid moiety of NAD⁺ can be synthesized from tryptophan using the kynurenine pathway or incorporated directly using nicotinate phosphoribosyl transferase (NPT1). We have identified the genes that encode the enzymes of the kynurenine pathway and for BNA5 (YLR231c) and BNA6 (YFR047c) confirmed that they encode kynureninase and quinolinate phosphoribosyl transferase respectively. We show that deletion of genes encoding kynurenine pathway enzymes are co-lethal with the Δnpt1, demonstrating that no other pathway for the synthesis of nicotinic acid exists in S. cerevisiae. Also, we show that under anaerobic conditions S. cerevisiae is a nicotinic acid auxotroph.

【 授权许可】

Unknown