Effect of heparin on phosphorylation site specificity of neuronal Cdc2‐like kinase

FEBS Letters

Qi, Zhong¹  Zhu, Xiaodong¹  Fujita, Donald J¹  Wang, Jerry H¹  Goedert, Michel² 

[1] Department of Medical Biochemistry, University of Calgary, Calgary, Alta T2N 4N1, Canada;MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK

关键词: Neuronal Cdc2-like kinase;    Tau;    Heparin;    Alzheimer's disease;    DTT;    dithiothreitol;    EDTA;    ethylenediaminetetraacetic acid;    GST;    glutathione S-transferase;    MOPS;    4-morpholinepropane sulfonic acid;    PBS;    phosphate buffered saline;   

DOI  :  10.1016/S0014-5793(98)00096-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Neuronal Cdc2-like kinase (Nclk) can be stimulated by heparin in a substrate-dependent manner. While phosphorylation of tau is markedly enhanced by heparin, phosphorylation of histone H1 by Nclk is essentially unaffected. A histone H1 peptide, HS(9–18): PKTPKKAKKL, and its substitution analogues were used to examine the basis of the differential heparin effect. In the presence of heparin, the phosphorylation site specificity of Nclk is altered and only proline immediately following the phosphorylation site is still an essential substrate determinant. This change in the site specificity may adequately account for the differential heparin effect on histone H1 and tau phosphorylation.

【 授权许可】

Unknown