Protein synthesis is involved in the modulation of the level of the phosphoenolpyruvate carboxykinase mRNA by changes in cell volume in isolated rat hepatocytes

FEBS Letters

Chedeville, Arlette¹  Quillard, Muriel¹  Fairand, Alain¹  Husson, Annie¹  Lavoinne, Alain¹ 

[1]Groupe de Biochimie et Physiopathologie Digestive et Nutritionnelle (GBPDN), Institut Fédératif de Recherches Multidisciplinaires sur les Peptides No. 23 (IFRMP), UFR Médecine-Pharmacie de Rouen, Avenue de l'Université, P.O. Box 97, 76803 Saint Etienne du Rouvray Cedex, France

关键词: Hydration state;    Hepatocyte;    Phosphoenolpyruvate carboxykinase mRNA;    Protein synthesis;   

DOI  :  10.1016/S0014-5793(98)00076-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The mechanism of action of hydration state was studied on phosphoenolpyruvate carboxykinase (PCK) gene expression in isolated rat hepatocytes. Hypoosmolarity decreased the level of the PCK mRNA after a lag period of about 60 min. The decreasing effect of hypoosmolarity was totally blocked by inhibitors of both protein synthesis and gene transcription. Moreover, hypoosmolarity specifically increased the synthesis of a 45 000 M _r protein, which decreased in the presence of inhibitors of transcription. A close relationship between the synthesis of the 45 000 M _r protein and the decrease in the PCK mRNA level was observed, suggesting that this protein might potentially be involved in the regulation of the level of the PCK mRNA by cell swelling.

【 授权许可】

Unknown