Hypoosmolarity and glutamine increased the β‐actin gene transcription in isolated rat hepatocytes

FEBS Letters

Chedeville, Arlette¹  Quillard, Muriel¹  Fairand, Alain¹  Husson, Annie¹  Lavoinne, Alain¹ 

[1]Groupe de Biochimie et Physiopathologie Digestive et Nutritionnelle (GBPDN), Institut Fédératif de Recherches Multidisciplinaires sur les Peptides No. 23 (IFRMP), U.F.R. Médecine-Pharmacie de Rouen, P.O. Box 97, 76803 Saint-Etienne-du-Rouvray Cedex, France

关键词: Hydration state;    Glutamine;    Hepatocyte;    Transcription;    β-Actin;   

DOI  :  10.1016/0014-5793(96)00985-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The mechanism of action of hydration state was studied on β-actin gene expression in isolated hepatocytes. Results obtained with Northern blot analysis and run on transcription assays show that hypoosmolarity increased and hyperosmolarity decreased the β-actin mRNA level through a corresponding modulation of the rate of the gene transcription. Glutamine, which is known to induce cell swelling, also increased the β-actin mRNA level in a dose-dependent manner and induced a stimulation of the β-actin gene transcription. Thus, cell hydration state regulates gene expression in the liver through a transcriptional mechanism.

【 授权许可】

Unknown