Regulation of osteopontin expression in rat mesangial cells

FEBS Letters

Pröls, Felicitas¹  Marx, Martin¹  Heidgress, Dorothe¹  Rupprecht, Harald D¹ 

[1] Medizinische Klinik IV, Universität Erlangen-Nürnberg, 91054 Erlangen, Germany

关键词: Differential display;    Osteopontin;    Proliferation;    Differentiation;    Rat mesangial cell;    DMEM;    Dulbecco's modified Eagle's medium;    DTAF;    dichlorotriazinyl amino fluorescein;    FCS;    fetal calf serum;    M;    adenine;    cytosine;    or guanine;    PBS;    phosphate-buffered saline;    PCR;    polymerase chain reaction;    PDGF;    platelet-derived growth factor;   

DOI  :  10.1016/S0014-5793(97)01587-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hypercellularity and accumulation of extracellular matrix are common responses of renal glomeruli to inflammatory stimuli. Using the differential display approach, we compared the gene expression patterns of proliferating and differentiating rat mesangial cells in two- and three-dimensional cultures. Osteopontin, an extracellular matrix protein, was found to be transcribed, synthesized, and secreted by rat mesangial cells. Osteopontin transcription was not associated with cell proliferation and was found to be FCS-inducible in proliferating cells. Osteopontin expression was independent of exogenously supplied FCS in differentiating cells. The presented data indicate that osteopontin is differentially regulated in proliferating and differentiating mesangial cells.

【 授权许可】

Unknown