The differentiation‐related gene 1, Drg1, is markedly upregulated by androgens in LNCaP prostatic adenocarcinoma cells

FEBS Letters

Verhoeven, Guido¹  Swinnen, Johannes V.¹  Heyns, Walter¹  Ulrix, William¹ 

[1] Laboratory for Experimental Medicine and Endocrinology, Faculty of Medicine, Onderwijs en Navorsing, Gasthuisberg, Catholic University of Leuven, Herestraat 49, B-3000 Leuven, Belgium

关键词: Differential display;    Differentiation;    Prostate cancer;    Androgen;   

DOI  :  10.1016/S0014-5793(99)00845-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A differential display technique was used to identify androgen-regulated genes in LNCaP prostatic adenocarcinoma cells. One of the genes markedly upregulated by androgens proved to be identical to differentiation-related gene 1 (Drg1; also described as RTP, Cap43 and rit42), a gene whose expression has recently been shown to be diminished in colon, breast and prostate tumors. We show that Drg1 is abundantly expressed in the (androgen-exposed) human prostate and that its expression is stimulated some 14-fold in androgen-treated LNCaP cells. The ligand specificity of the induction reflects the altered specificity of the mutated androgen receptor in LNCaP. In androgen receptor negative tumor lines basal expression is slightly higher than in LNCaP but inducibility is absent. These data suggest that Drg1 is a novel marker of androgen-induced differentiation in the human prostate.

【 授权许可】

Unknown