Regulated expression of mature human insulin in the liver of transgenic mice

FEBS Letters

Mignon, Alexandre¹  Porteu, Arlette¹  Mitanchez, Delphine¹  Chen, Ruihuan¹  Bertagna, Xavier²  Massias, Jean-Francis²  Kahn, Axel¹ 

[1] INSERM U129, Institut Cochin de Génétique Moléculaire, Faculté Cochin-Port Royal, 75014 Paris, France;Groupe d'Etude en Physiopathologie Endocrinienne (GEPE), Paris, France

关键词: Insulin;    Furin;    L-type pyruvate kinase gene;    Transgenic mice;    Insulin-dependent diabetes mellitus;   

DOI  :  10.1016/S0014-5793(97)01574-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Transgenic mice expressing either human proinsulin cDNA or mutated proinsulin cDNA in the liver were created. The human proinsulin cDNA was mutated to generate a protein cleavable by the ubiquitous prohormone convertase furin, thus leading to mature insulin peptide. All transgenic lines expressed human C-peptide in the blood, whose level varied according to nutritional conditions. High performance liquid chromatography fractionation of mouse serum revealed that mutant proinsulin was effectively processed into mature insulin in vivo. This transgenic mouse model provides a useful tool for further prospects of gene therapy of insulin-dependent diabetes mellitus.

【 授权许可】

Unknown