FEBS Letters | |
Identification of an atypical lipoprotein‐binding protein from human aortic smooth muscle as T‐cadherin | |
Molokoedov, Alexander S1  Tkachuk, Vsevolod A3  Stambolsky, Dmitry V3  Sidorova, Maria V1  Philippova, Maria P3  Spirov, Valentin G4  Kuzmenko, Elena S2  Bochkov, Valery N3  Resink, Therese J2  | |
[1] Laboratory of Peptide Synthesis, Institute of Experimental Cardiology, Cardiology Research Center, 121552 Moscow, Russia;Laboratory of Hypertension, Department of Research, Basel University Hospitals, 4031 Basel, Switzerland;Laboratory of Molecular Endocrinology, Institute of Experimental Cardiology, Cardiology Research Center, 121552 Moscow, Russia;Laboratory of Protein Engineering, Institute of Experimental Cardiology, Cardiology Research Center, 121552 Moscow, Russia | |
关键词: Lipoprotein; Cadherin; Vascular smooth muscle cell; VSMC; vascular smooth muscle cell; LDL; low density lipoprotein; mAb; monoclonal antibody; IEF; isoelectric focusing; 2-D; two-dimensional; GPI; glycosylphosphatidylinositol; | |
DOI : 10.1016/S0014-5793(97)01562-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We have previously described an atypical lipoprotein-binding protein of about 105 kDa (p105) in membranes of vascular smooth muscle cells (VSMCs) that is distinct from currently known lipoprotein receptors. In the present work we have developed a procedure for purification of p105 from human aortic media. Partial sequencing of purified protein has revealed identity of p105 with human T-cadherin. Anti-peptide antisera raised against human T-cadherin recognized a protein spot corresponding to the purified p105 on two-dimensional Western blots. The antisera also inhibited LDL binding to p105 on ligand blots. We conclude that the 105 kDa lipoprotein-binding protein present in human VSMCs is T-cadherin, an unusual glycosylphosphatidylinositol-anchored member of the cadherin family of cell-cell adhesion proteins.
【 授权许可】
Unknown
【 预 览 】
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