Vasoconstrictor‐induced protein‐tyrosine phosphorylation in cultured vascular smooth muscle cells

FEBS Letters

Ishida, Yoshihiro²  Shii, Kozui¹  Kawahara, Yasuhiro²  Koide, Masanobu²  Tsuda, Terutaka²  Yokoyama, Mitsuhiro² 

[1] Hyogo Institute for Research in Adult Diseases, Akashi 673, Japan;Department of Internal Medicine (1st Division), Kobe University School of Medicine, Kobe 650, Japan

关键词: Vasoconstrictor;    Tyrosine phosphorylation;    Protein kinase C;    Calcium;    Vascular smooth muscle cell;    VSMC;    vascular smooth muscle cells;    ang II;    angiotensin 11;    AVP;    [Arg]vasopressin;    5HT;    5-hydroxytryptamine;    NE;    norepinephrine;    PMA;    phorbol 12-myristate 13-acetate;    PDBu;    phorbol 12;    13-dibutyrate;    4α-PDD;    4α-phorbol 12;    13-didecanoate;    PDGF;    platelet-derived growth factor;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(91)80721-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In cultured rat aortic smooth muscle cells, angiotensin II induced tyrosine phosphorylation of at least 9 proteins with molecular masses of 190, 117, 105, 82, 79, 77, 73, 45 and 40 kDa in time- and dose-dependent manners. Other vasoconstrictors such as [Arg]vasopressin, 5-hydroxytryptamine and norepinephrine induced the tyrosine phosphorylation of the same set of proteins as angiotensin II. The tyrosine phosphorylation of these proteins was mimicked by the protein kinase C-activating phorbol ester, phorbol 12 myristate, 13-acetate, and the Ca²⁺ ionophore, ionomycin. These results demonstrate that the vasoconstrictors stimulate the tyrosine phosphorylation of several proteins in vascular smooth muscle cells and suggest that the tyrosine phosphorylation reactions are the events distal to the activation of protein kinase C and Ca²⁺ mobilization in the intracellular signalling pathways of the vasoconstrictors.

【 授权许可】

Unknown