Cytokine‐induced expression of an inducible type of nitric oxide synthase gene in cultured vascular smooth muscle cells

FEBS Letters

Kawahara, Yasuhiro¹  Koide, Masanobu¹  Tsuda, Terutaka¹  Yokoyama, Mitsuhiro¹ 

[1] Department of Internal Medicine (1st Division), Kobe University School of Medicine, Kobe 650, Japan

关键词: Nitric oxide;    Nitric oxide synthase;    Cytokine;    Interferon γ;    Tumor necrosis factor α;    Vascular smooth muscle cell;    NO;    nitric oxide;    NOS;    nitric oxide synthase;    IL1;    interleukin 1;    TNFα;    tumor necrosis factor α;    IFNγ;    interferon γ;    VSMC;    vascular smooth muscle cells;    iNOS;    inducible nitric oxide synthase;    LPS;    lipopolysaccharide;    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;   

DOI  :  10.1016/0014-5793(93)80514-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In unstimulated cultured vascular smooth muscle cells (VSMC), mRNA of an inducible macrophage-type of nitric oxide synthase (iNOS) was barely detectable. Interferon γ (IFNγ) and tumor necrosis factor α (TNFα) markedly increased iNOS mRNA levels in time- and dose-dependent manners. The induction of iNOS mRNA paralleled the cytokine-induced nitrite production. Actinomycin D abolished the IFNγ- and TNFγ-induced increases in iNOS mRNA and nitrite production. Cycloheximide, which abolished both the IFNγ- and TNFα-induced increases in nitrite production, had no effect on the IFNγ-induced increase in iNOS mRNA but markedly inhibited the TNFα-induced one. These results suggest that IFNγ directly induces the expression of the iNOS gene whereas TNFα mainly induces it via the induction of an intermediary protein in cultured VSMC.

【 授权许可】

Unknown