FEBS Letters | |
The nitric oxide synthase of mouse spermatozoa | |
Viggiano, J.M1  Goin, J.C1  Polak, J.M2  Canteros, M.G1  Perez Martinez, S1  Herrero, M.B1  Franchi, A.M1  Boquet, M1  Gimeno, M.A.F1  | |
[1] Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Serrano 669 (1414), Buenos Aires, Argentina;Hammersmith Hospital, Du Cane Road, London W12 0NN, UK | |
关键词: Nitric oxide synthase; Western blot; Nitric oxide; Spermatozoa; (Mouse); | |
DOI : 10.1016/S0014-5793(97)00570-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Nitric oxide synthase (NOS) was evidenced in mature mouse spermatozoa by means of biochemical techniques and Western blot. During 120 min of incubation, 107 spermatozoa synthesized 7±2 pmol of l-[14C]citrulline. Besides, l-citrulline formation depended on the incubation time and on the concentration of l-arginine present in the incubation medium. Different concentrations of N G-nitro-l-arginine methyl ester (L-NAME) but not aminoguanidine, inhibited l-[14C]citrulline formation. Western-blot analysis of solubilized sperm proteins revealed a unique band of M r=140 kDa with the neural, endothelial and inducible NOS antisera tested. These results provide evidence that mature mouse sperm contains a NOS isoform and that spermatozoa have the potential ability to synthesize NO, suggesting a role for endogenous NO on mammalian sperm function.
【 授权许可】
Unknown
【 预 览 】
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