Induction of apoptosis and of interleukin‐1β secretion by 7β‐hydroxycholesterol and 7‐ketocholesterol: partial inhibition by Bcl‐2 overexpression

FEBS Letters

Lemaire, Stéphanie¹  Lizard, Gérard¹  Néel, Dominique¹  Gambert, Philippe¹  Gueldry, Serge¹  Monier, Serge¹ 

[1] Laboratoire de Biochimie des Lipoprotéines, INSERM CJF 93-10, Faculté de Médecine, 7 Bd Jeanne d'Arc, 21033 Dijon, France

关键词: Oxysterol;    Apoptosis;    Bcl-2;    IL-1β;   

DOI  :  10.1016/S0014-5793(97)01473-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The oxysterols, 7β-hydroxycholesterol and 7-ketocholesterol, are involved in the cytotoxicity of oxidized LDL. To elucidate their molecular mechanisms, the human promonocytic leukemia cells U937 and U4 were used. U4 cells overexpressing Bcl-2 were obtained by transfection of U937 cells. 7β-hydroxycholesterol and 7-ketocholesterol induced nuclear condensation and/or fragmentation, internucleosomal DNA fragmentation, and IL-1β secretion, which were partially inhibited by Bcl-2 overexpression. These findings underline that these oxysterols could constitute major risk factors in atherosclerosis by their cytotoxicity and their ability to induce IL-1β release which might favor the recruitment of immunocompetent cells in the atherosclerotic plaque.

【 授权许可】

Unknown