Inhibition of caspase‐1 induces cell death in pancreatic carcinoma cells and potentially modulates expression levels of bcl‐2 family proteins

FEBS Letters

Schlosser, Sophia¹  Ramadani, Marco¹  Gansauge, Susanne¹  Beger, Hans-Günther¹  Gansauge, Frank¹ 

[1] Division of Molecular Oncology, Department of General Surgery, University of Ulm, Steinhövelstraße 9, 89075 Ulm, Germany

关键词: Caspase-1;    Epidermal growth factor;    Bcl-2;    Apoptosis;    Necrosis;    Pancreatic carcinoma;    ICE;    interleukin-1β-converting enzyme (caspase-1);    IL-1β;    interleukin-1β;    EGF;    epidermal growth factor;    p-YVAD.CHO (caspase-1 inhibitor I);    Ac-AAVALLPAVLLALLAP-YVAD.CHO (acetyl-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Tyr-Val-Ala-Asp-aldehyde);    p-LEVD.CHO (caspase-4 inhibitor I);    Ac-AAVALLPAVLLALLAP-LEVD.CHO (acetyl-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Leu-Glu-Val-Asp-aldehyde);   

DOI  :  10.1016/S0014-5793(01)02144-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Caspase-1 (interleukin-1β-converting enzyme) is reported to play an important role in the regulation of apoptosis. We investigated the inhibition of caspase-1 by the cell permeable caspase-1 inhibitor Ac-AAVALLPAVLLALLAP-YVAD.CHO in pancreatic carcinoma cells. Inhibition of caspase-1 induced a non-apoptotic/‘necrotic-like’ cell death in AsPC-1, BxPC-3, MiaPaCa-2 and Panc-1 cells. Expression levels of bcl-2 and bax were up-regulated in caspase-1 inhibitor-treated cells while that of bcl-x_L remained unaltered. Our observations support our previous findings that caspase-1 is potentially involved in anti-apoptotic processes in pancreatic carcinoma.

【 授权许可】

Unknown