Cooperativity of phosphatidylinositol transfer protein and phospholipase D in secretory vesicle formation from the TGN – phosphoinositides as a common denominator?

FEBS Letters

Huttner, Wieland B¹  Lorra, Christoph¹  Tüscher, Oliver¹  Bouma, Barend²  Wirtz, Karel W.A² 

[1] Department of Neurobiology, University of Heidelberg, Im Neuenheimer Feld 364, D-69120 Heidelberg, Germany;Centre for Biomembranes and Lipid Enzymology, Utrecht University, PO Box 80054, NL-3508 TB Utrecht, The Netherlands

关键词: Aminoglycoside;    Phosphatidylinositol transfer protein;    Phospholipase D;    Phosphoinositide;    Secretory vesicle;    Trans-Golgi network;    ARF;    ADP-ribosylation factor;    C6-NBD-PC;    1-palmitoyl-2-[6-[(7-nitro-2-1;    3-benzoxadiazol-4-yl)amino]caproyl]-sn-glycero-3-phosphocholine;    C6-NBD-PA;    1-palmitoyl-2-[6-[(7-nitro-2-1;    3-benzoxadiazol-4-yl)amino]caproyl]-sn-glycero-3-phosphate;    CSVs;    constitutive secretory vesicles;    hsPG;    heparan sulfate proteoglycan;    ISGs;    immature secretory granules;    PI;    phosphatidylinositol;    PIP2;    phosphatidylinositol bis phosphate;    PA;    phosphatidic acid;    PC;    phosphatidyl choline;    PITP;    phosphatidylinositol transfer protein;    PLD;    phospholipase D;    PNS;    post-nuclear supernatant;    SgII;    secretogranin II;    TGN;    trans-Golgi network;   

DOI  :  10.1016/S0014-5793(97)01471-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphatidylinositol transfer protein (PITP) and phospholipase D (PLD) stimulate the formation of constitutive secretory vesicles (CSVs) and immature secretory granules (ISGs) from the trans-Golgi network (TGN) in a cell-free system. The stimulatory effects of PITP and PLD are additive. Stimulation by either PITP or PLD is blocked by geneticin, a member of the aminoglycoside antibiotics known to bind to phosphoinositides. Since the PLD we used is insensitive to geneticin, our results suggest that phosphoinositides promote secretory vesicle formation as downstream effectors of both PITP and PLD, possibly via the recruitment of proteins mediating membrane budding and fission.

【 授权许可】

Unknown