Calcium‐dependence of hydrogen peroxide‐induced c‐fos expression and growth stimulation of multicellular prostate tumor spheroids

FEBS Letters

Sauer, Heinrich¹  Diedershagen, Heike¹  Hescheler, Jürgen¹  Wartenberg, Maria¹ 

[1] Institute for Neurophysiologie, University of Cologne, Robert-Koch Str. 39, D-50931 Cologne, Germany

关键词: Hydrogen peroxide;    Calcium;    P2-purinoreceptor;    c-fos;    Tumor growth;    ROS;    reactive oxygen species;    BAPTA;    1;    2-bis(o-aminophenoxy)ethane-N;    N;    N′;    N′-tetraacetic acid;    NFκB;    nuclear factor κB;    ERK2;    extracellular signal-regulated protein kinase;    MAPK;    mitogen-activated protein kinase;    EDTA;    ethyleneglycoltetraacetic acid;    HEPES;    N-[2-hydroxyethyl]piperazine-N′-[2-ethanesulfonic acid];   

DOI  :  10.1016/S0014-5793(97)01456-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hydrogen peroxide (H₂O₂) in nanomolar concentrations (20–100 nM) stimulated the growth of small (diameter 100±30 μm) multicellular prostate cancer spheroids and increased c-fos expression. H₂O₂ transiently raised [Ca²⁺]_i by Ca²⁺ release from intracellular stores as the transient persisted in low (10 nM) Ca²⁺ solution but was abolished when intracellular Ca²⁺ stores were depleted by thapsigargin or chelation of [Ca²⁺]_i with BAPTA. The H₂O₂-induced [Ca²⁺]_i transient was furthermore inhibited by the P₂-purinoreceptor antagonists suramin and basilen blue, indicating that H₂O₂ may act via purinergic receptor stimulation. Treatment of spheroids with either suramin, basilen blue or BAPTA inhibited the H₂O₂-induced growth stimulation and c-fos expression, indicating that the H₂O₂-mediated growth stimulation of multicellular spheroids is mediated via a Ca²⁺-dependent pathway.

【 授权许可】

Unknown