A novel lipophilic spin probe for the measurement of radiation damage in mouse brain using in vivo electron spin resonance (ESR)

FEBS Letters

Anzai, Kazunori²  Miura, Yuri²  Ozawa, Toshihiko²  Takahashi, Sentaro¹ 

[1] Environmental and Toxicological Sciences Research Group, National Institute of Radiological Sciences, Anagawa 4-9-1, Inage-ku, Chiba-shi 263, Japan;Department of Bioregulation Research, National Institute of Radiological Sciences, Anagawa 4-9-1, Inage-ku, Chiba-shi 263, Japan

关键词: In vivo electron spin resonance;    Nitroxyl radical;    Brain;    Radiation;    Blood-brain barrier;    Free radical;   

DOI  :  10.1016/S0014-5793(97)01443-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

As a possible lipophilic spin probe of in vivo electron spin resonance (ESR), 3-methoxy carbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-yloxy (MCPROXYL) was examined. The permeability of the blood-brain barrier to this compound was evaluated with a brain uptake index and autoradiography, with result that this probe is well distributed in the brain. The in vivo ESR spectra were measured in the head and the abdomen of MCPROXYL-injected living mice. The rate of signal decay of MCPROXYL in the head measured at one hour after X-irradiation was about 75% of that of the controls. The decrease in the head seems to be related to the early response of the brain to X-irradiation. This is the first report that the behavior of free radical such as MCPROXYL in the brain is influenced by X-irradiation. MCPROXYL is thus useful as a novel spin probe for in vivo ESR to monitor the radiation damage in the brain.

【 授权许可】

Unknown