Cloning of rat uncoupling protein‐3 and uncoupling protein‐2 cDNAs: their gene expression in rats fed high‐fat diet

FEBS Letters

Hosoda, Kiminori²  Yoshimasa, Yasunao²  Itoh, Hiroshi²  Yamori, Yukio¹  Tanaka, Tokuji²  Nishimura, Haruo²  Inoue, Gen²  Nakao, Kazuwa²  Son, Cheol²  Doi, Kentaro²  Fukunaga, Yasutomo²  Matsuda, Junichi² 

[1] Department of Life Science, Kyoto University Graduate School of Human and Environmental Studies, Kyoto 606, Japan;Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606, Japan

关键词: Uncoupling protein;    Skeletal muscle;    Energy expenditure;    High-fat diet;    Obesity;    Brown adipose tissue;   

DOI  :  10.1016/S0014-5793(97)01381-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In order to elucidate energy balance in the skeletal muscle, we cloned cDNA of a homologue of uncoupling protein (UCP) from rat skeletal muscle. We also cloned rat UCP-2 cDNA from rat brown adipose tissue (BAT). The UCP cloned from rat skeletal muscle showed 57% and 72% identity with rat UCP-1 and UCP-2. The mRNA was expressed abundantly in the skeletal muscle, moderately in the BAT, and slightly in the white adipose tissue (WAT) with a major band at 2.5 kb and a minor band at 2.8 kb, while the UCP-2 gene expression was widely detected in the whole body with substantial levels in the WAT and with slight levels in the skeletal muscle and BAT. The rat UCP cloned in the present study showed 86% identity with the recently cloned human UCP-3, which was also expressed abundantly in the skeletal muscle with a signal of 2.4 kb. Therefore, the rat UCP was considered to be rat UCP-3. In rats fed high-fat diet the UCP-3 gene expression was augmented 2-fold in the skeletal muscle while UCP-2 mRNA levels were increased significantly (1.6-fold) in the epididymal WAT. Augmented expression of UCPs may provide defense against high-fat induced obesity and impairment of glucose metabolism.

【 授权许可】

Unknown