Selective activation of JNK/SAPK by interleukin‐1 in rabbit liver is mediated by MKK7

FEBS Letters

Holland, Pamela¹  Cooper, Jonathan¹  Kracht, Michael³  Saklatvala, Jeremy²  Finch, Andrew² 

[1] Fred Hutchinson Cancer Research Center, A2-025, Weintraub Basic Sciences Bldg. 1100 Fairview Ave. N., Seattle, WA 98109, USA;Kennedy Institute of Rheumatology, 1 Aspenlea Rd, Hammersmith, London W6 8LH, UK;Medical School Hannover, Institute for Molecular Pharmacology, Carl Neuberg Straße 1, D-30625 Hannover, Germany

关键词: Interleukin-1;    Mitogen-activated protein kinase;    Jun N-terminal kinase;    Stress-activated protein kinase;    Mitogen-activated protein kinase kinase 7;    Liver;    JNK/SAPK;    jun N-terminal kinase/stress-activated protein kinase;    GST;    glutathione S-transferase;    IL-1;    interleukin-1;    MAPK;    mitogen-activated protein kinase;    MKK;    MAPK kinase;    MKKK;    MAPKK kinase;   

DOI  :  10.1016/S0014-5793(97)01364-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Activation of jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) by interleukin-1 (IL-1) has been reported in many cells and in rabbit liver. Here we report selective activation of JNK/SAPK, without activation of p38 or p42 mitogen-activated protein kinases (MAPKs), by IL-1 in rabbit liver. We identified an IL-1 regulated JNK/SAPK activator present in rabbit liver using S Sepharose chromatography. It was purified and immunoprecipitated by two antisera to MAP kinase kinase 7 (MKK7). It was not recognised by an antibody to MKK4. We conclude that MKK7 is the activator of JNK/SAPK activated by IL-1 in liver and that JNK/SAPK is the only MAPK activated by IL-1 in liver.

【 授权许可】

Unknown